Bacillus subtilis Mutant Altered in Spore Morphology and in RNA Polymerase Activity
نویسندگان
چکیده
منابع مشابه
Bacillus subtilis spore coat.
In response to starvation, bacilli and clostridia undergo a specialized program of development that results in the production of a highly resistant dormant cell type known as the spore. A proteinacious shell, called the coat, encases the spore and plays a major role in spore survival. The coat is composed of over 25 polypeptide species, organized into several morphologically distinct layers. Th...
متن کاملA deoxyribonucleic acid polymerase I-deficient mutant of Bacillus subtilis.
A methyl methanesulfonate sensitive mutant of Bacillus subtilis appears to lack the enzyme DNA polymerase I. The absence of polymerase I permits the detection of a second DNA polymerizing enzyme in the mutant. This second polymerase is inhibitable by sulfhydryl antagonists, and shows maximal activity on double-stranded DNA substrates with many single strand breaks. Poly[d(A-T)] is less efficien...
متن کاملSigma factor displacement from RNA polymerase during Bacillus subtilis sporulation.
As Bacillus subtilis proceeds through sporulation, the principal vegetative cell sigma subunit (sigma(A)) persists in the cell but is replaced in the extractable RNA polymerase (RNAP) by sporulation-specific sigma factors. To explore how this holoenzyme changeover might occur, velocity centrifugation techniques were used in conjunction with Western blot analyses to monitor the associations of R...
متن کاملSpore Formation by Bacillus subtilis in Peptone Solutions Altered by Treatment with Activated Charcoal.
Some years ago it was found that Bacillus subtilis sporulated more profusely in Bacto-peptone broth when a small quantity of activated charcoal was added to the medium. Further studies revealed that although the charcoal was removed from the medium prior to inoculation, the percentage of cells sporulating during short incubation periods was still appreciably greater than in untreated Bacto-pept...
متن کاملProperties of the Bacillus subtilis spore coat.
About 70% of the protein in isolated Bacillus subtilis spore coats was solubilized by treatment with a combination of reducing and denaturing agents at alkaline pH. The residue, consisting primarily of protein, was insoluble in a variety of reagents. The soluble proteins were resolved into at least seven bands by sodium dodecyl sulfate gel electrophoresis. About one-half of the total was four p...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1970
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.66.2.404